Advertisement
You are prohibited from using or uploading content you accessed through this website into external applications, bots, software, or websites, including those using artificial intelligence technologies and infrastructure, including deep learning, machine learning and large language models and generative AI.
You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Prostate & Genitalia II1 Apr 2016

MP36-14 UPREGULATION OF PROSTATIC CANNABINOID RECEPTOR TYPE 2 FOLLOWING CASTRATION AND CAPSAICIN-INDUCED PROSTATITIS IN THE RATS

    View All Author Information

    INTRODUCTION AND OBJECTIVES

    The etiology of Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is unknown, and there are still no established treatments that consistently relieve patients’ symptoms. Endocannabinoids serve as important modulators of tissue inflammation and pain perception. And manipulation of cannabinoid system had been shown to reduce inflammation and lessen pain perception in some inflammatory disorders. This study investigated the changes of cannabinoid system following capsaicin-induced prostatitis in castrated and non-castrated rats.

    METHODS

    In adult male Sprague-Dawley rats androgen deprivation was induced with bilateral orchiectomy (OX). At 4 weeks after bilateral OX or sham operation, prostate inflammation was induced by intraprostatic capsaicin injection. Control group received intraprostatic vehicle injection. The expression of cannabinoid receptor type 1(CB1) and 2(CB2), fatty amide hydrolase (FAAH) and cyclooxygenase 2(COX-2) in each group were examined with RT-PCR for mRNA and immunohistochemistry. There are total 4 groups of animals with 6 to 8 animals in each group.

    RESULTS

    Capsaicin injection induced an inflammatory reaction with infiltration of leukocytes. Expression of COX-2 was enhanced following capsaicin injection. Capsaicin injection increased CB2 expression, while the expression of CB1 was not changed. Expression of FAAH, a endocannabinoid degradation enzyme, was reduced following capsaicin injection. Castration induced an atrophic change of the prostate gland. Castration enhanced the expression of CB1,CB2 and FAAH. Capsaicin injection also induced an inflammatory reaction in the castrated-prostate, but with a lesser degree of leukocyte accumulation as compared with non-castrated group. In the castrated-prostate capsaicin injection induced a further increase of CB2 expression, while expression of CB1 and FAAH was not changed.

    CONCLUSIONS

    The present study demonstrated a consistent up-regulation of CB2 following capsaicin-induced prostatitis in castrated and non-castrated rats. CB2 can be a promising therapeutic target in treating non-bacterial prostatitis.

    Advertisement