MP05-11 INNOVATIVE URETERAL STENT DEVELOPMENT USING NOVEL BIO-LIKE MATERIALS WITH ABILITY TO PREVENT CRYSTAL ADHESION IN VITRO AND IN VIVO
INTRODUCTION AND OBJECTIVE:
Ureteral stents used to prevent ureteral obstruction in urological patients present the challenge of ureteral stent obstruction due to urinary crystal adhesion. There is active research into the development of ureteral stents with surface coatings and smoothing of the surface to reduce foreign body adhesion. In this study, the novel ureteral stent coated with polydopamine (PDA), which is formed by the polymerization of dopamine and close to the substances existed in human body and so has a low recognition reaction as a foreign substance, were investigated for ability to prevent crystal (Calcium: Ca) adhesion.
The ureteral stent was incubated in artificial urine at 37°C for 3 weeks, and the amount of Ca deposition on the surface of the ureteral stent was compared between the PDA-coated stent and commercially available stents by microscopic observation and Inductively Coupled Plasma (ICP) analysis. As to in vivo study, in rat model of hypercalciuria induced by drinking ethylene glycol and ammonium chloride, stents were implanted in the bladder for one month and Ca deposition on the surface of the ureteral stent was determined.
After immersion in artificial urine for 3 weeks, the PDA-coated ureteral stent showed less Ca deposition on the stent surface than the commercially available ureteral stent (p=0.0468). There was no significant difference in the increase in pH of the artificial urine in which the ureteral stent was immersed between the PDA-coated and commercially available ureteral stents. With respect to the rat experimental model with Ca including-urine, the PDA-coated ureteral stent had less Ca deposition on the stent surface than the commercially available stent (p=0.0412). No apparent safety-related adverse events were observed.
Our novel PDA-coated ureteral stent showed less amount of Ca deposition on the stent surface than commercial stents in artificial urine. The experimental rat model with Ca including-urine also showed a similar data of less Ca deposition on the stent surface than commercial stent, suggesting that this novel stent is less likely to cause stent occlusion (WO/2022/176834).
Source of Funding:
This study was supported by the Uehara Memorial Foundation, a Grant-in-Aid for Research Activity Start-up (20K22979) from the Japan Society for the Promotion of Science (JSPS) and Suzuken Memorial Foundation (20-014)