A Monoclonal Antibody Identifies Vimentin Filaments in Sertoli Cells and in a Subset of Epithelial Cells in the Rat Epididymis, Urinary Bladder, and Prostate
Abstract
Purpose:
We describe a monoclonal antibody (Mab B2) against vimentin filaments in rat Sertoli cells and in a subset of epithelial cells in the rat epididymis, prostate and urinary bladder.
Materials and Methods:
The immunoreactivity of Mab B2 was examined on cryostat sections and immunoblots and compared with the reactivity of a previously characterized monoclonal antibody against vimentin.
Results:
While both antibodies recognized vimentin in Sertoli cells, only Mab B2 recognized vimentin in urogenital tract epithelia.
Conclusions:
Vimentin is expressed in the urogenital tract, and differential reactivities of antibodies may be responsible for conflicting results reported elsewhere for vimentin expression.
References
- 1 : Cytokeratin polypeptide patterns of different epithelia of the human urogenital tract.. J. Histochem. Cytochem.1985; 33: 415. Google Scholar
- 2 : Monoclonal antibodies specific for subsets of epidermal keratins: biochemical and immunocytochemical characterization-applications in pathology and cell culture.. J. Pathol.1985; 145: 341. Google Scholar
- 3 : Changes in the distribution of microtubules and intermediate filaments in mammalian Sertoli cells during spermatogenesis.. Anat. Rec.1988; 220: 143. Google Scholar
- 4 : Intermediate-sized filaments present in Sertoli cells are of the vimentin type.. Eur. J. Cell Biol.1979; 19: 269. Google Scholar
- 5 : Selective expression of cytokeratin polypeptides in various epithelia of human Brenner tumor.. Hum. Pathol.1988; 19: 640. Google Scholar
- 6 : Co-expression of cytokeratin and vimentin filaments in rete testis and epididymis.. Virchows Archiv. A. Pathol. Anat.1991; 418: 119. Google Scholar
- 7 : Immunohistochemical investigation of different cytokeratins and vimentin in the human epididymis from the fetal period up to adulthood.. Cell Tissue Res.1989; 257: 661. Google Scholar
- 8 : Immunohistochemical investigation of different cytokeratins and vimentin in the prostate carcinoma.. Pathol. Res. Pract.1987; 182: 617. Google Scholar
- 9 : Germ cell binding to rat Sertoli cells in vitro.. Biol. Reprod.1987; 37: 1271. Google Scholar
- 10 : Pure Sertoli cell cultures.. A new model for the study of somatic-germ cell interactions. J. Androl.1981; 5: 249. Google Scholar
- 11 : Plasma membranes from rat Sertoli cells: purification and properties.. Biol. Reprod.1986; 35: 1235. Google Scholar
- 12 : Monoclonal Antibodies: Principles and Practice.. Orlando, Florida: Academic Press,1986: 25. Google Scholar
- 13 : The outer boundary of the cytoskeleton: a lamina derived from plasma membrane proteins.. Cell1979; 17: 859. Google Scholar
- 14 : High resolution two-dimensional electrophoresis of proteins.. J. Biol. Chem.1975; 250: 4007. Google Scholar
- 15 : Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.. Proc. Natl. Acad. Sci. U.S.A.1979; 76: 4350. Google Scholar
- 16 : Vimentin: a phosphoprotein under hormonal regulation.. J. Cell Biol.1981; 90: 803. Google Scholar
- 17 : Monoclonal antibodies: principles and practice.. Hum. Pathol.1986; 17: 212. Google Scholar
- 19 : Monoclonal antibody to a 43000 Mr surface protein of a human leukemia cell line (THP-1) crossreacts with the fibroblast intermediate filament protein vimentin.. J. Cell Sci.1985; 73: 87. Google Scholar
- 20 : The antigenic structure of proteins: a reappraisal.. Annu. Rev. Immunol.1984; 2: 67. Google Scholar
- 21 : Antibodies raised against a peptide corresponding to a Gs domain reveal a vimentin-associated protein.. J. Histochem. Cytochem.1993; 41: 709. Google Scholar
- 22 : Nonspecific cytotoxic cells in fish: antigenic cross-reactivity of a function associated molecule with the intermediate filament vimentin.. Cell. Immunol.1993; 148: 208. Google Scholar
- 23 : Identification of a vimentin-like function associated molecule (FAM) on rat NK cells: evidence for receptor function.. Scand. J. Immunol.1993; 37: 131. Google Scholar
- 24 : Development of cell types of regional differences in the postnatal rat epididymis.. Am. J. Anat.1979; 154: 27. Google Scholar
- 25 : Immunohistochemistry of the human ductus epididymis.. Anat. Rec.1993; 235: 560. Google Scholar
- 26 : Expression of keratin and vimentin intermediate filaments in rabbit bladder epithelial cells at different stages of benzo [a] pyrene-induced neoplastic progression.. J. Cell Biol.1981; 90: 63. Google Scholar
- 27 : Immunoanatomic distribution of cytostructural and tissue-associated antigens in the human urinary tract.. Am. J. Pathol.1987; 126: 269. Google Scholar
- 28 : Evidence for the blood-urine barrier depending on urothelium and carbonic anhydrase positive fibroblasts.. Lab. Invest.1987; 57: 535. Google Scholar
- 29 : The fine structure of the basal cell of the human prostate.. Lab. Invest.1966; 15: 1768. Google Scholar
- 30 : Basal cells of H-Dunning tumor myoepithelial cells.. Histochemistry1991; 95: 341. Google Scholar
- 31 : Pharmacologically induced ultrastructural and immunohistochemical changes in the prostate of the castrated dog.. Prostate1987; 11: 211. Google Scholar
- 32 : Sclerosing adenosis of the prostate gland.. Am. J. Surg. Pathol.1992; 16: 383. Google Scholar
- 33 : Multidirectional differentiation in the normal, hyperplastic, and neoplastic human prostate.. Hum. Pathol.1994; 25: 42. Google Scholar
- 34 : The proliferative function of basal cells in the normal and hyperplastic human prostate.. Prostate1994; 24: 114. Google Scholar
- 35 : Cell proliferation studies in the rat prostate.. II. The effects of castration upon androgen-induced regeneration upon basal and secretory cell proliferation. Prostate1987; 11: 339. Google Scholar
- 36 :
Applications of immunohistochemistry to the diagnosis and prognostication of prostate carcinoma and prostatic intraepithelial neoplasia . In: . Norwalk: Appleton and Lange1994: 303. Google Scholar - 37 : An alteration in the phosphorylation of vimentin-type intermediate filaments is associated with mitosis in cultured mammalian cells.. Cell1982; 29: 43. Google Scholar
- 38 : Follicle-stimulating hormone-dependent phosphorylation of vimentin in cultures of rat Sertoli cells.. Proc. Natl. Acad. Sci. U.S.A.1983; 80: 993. Google Scholar
- 39 : Retinoylation of vimentin in human myeloid leukemia cell line HL60.. J. Biol. Chem.1994; 269: 5913. Google Scholar
- 40 : Protein kinase C-induced redistribution of the cytoskeleton and phosphorylation of vimentin in cultured brain macrophages.. J. Neurosci. Res.1991; 29: 362. Google Scholar
- 41 : Differences in vimentin distribution in glial cell in culture revealed with an antibody against a phosphorylated epitope.. Int. J. Dev. Neuroscience1993; 11: 739. Google Scholar
- 42 : Cytoskeleton-associated plectin: in situ localization, in vitro reconstruction, and binding to immobilized intermediate filament proteins.. J. Cell Biol.1988; 106: 723. Google Scholar
- 43 : Molecular and cellular biology of intermediate filaments.. Annu. Rev. Biochem.1988; 57: 593. Google Scholar
- 44 : Association of v-mos with soluble vimentin in vitro and in transformed cells.. Oncogene1993; 8: 2207. Google Scholar
- 45 : A monoclonal antibody recognizes a form of intermediate filament protein in rat Sertoli cells that is not present in seminiferous peritubular cells.. Biol. Reprod.1986; 35: 227. Google Scholar
- 46 : Widespread occurrence of intermediate-sized filaments of the vimentin-type in cultured cells from diverse vertebrates.. Exp. Cell Res.1979; 123: 125. Google Scholar
- 47 : Tumor diagnosis by intermediate filament typing: a novel tool for surgical pathology.. Lab. Invest.1983; 48: 372. Google Scholar
- 48 : Expression of intermediate filaments and desmoplakin in vertebrate olfactory mucosa.. Anat. Rec.1988; 221: 754. Google Scholar
Departments of Cell Biology, Neurobiology and Anatomy, and Otolaryngology, The Ohio State University, Columbus, Ohio.